Discuss the techniques of gene cloning with a properly labelled diagram.
Answer variants:
Gene cloning
Inserting the DNA fragment into a suitable vector called plasmid to make rDNA
Clone
Isolating the desired DNA fragment using restriction enzymes
Stanley Cohen
Salmonella typhimurium
restriction enzymes
Selection and multiplication of recombinant host cell to obtain a clone
Brain
Expression of the cloned gene in a host cell
Incorporation of rDNA into the bacterial host cell
Herbert Boyer
a. is a carbon copy of an individual. It is a process of creating a genetically identical copy of an organism.
b. involves inserting a gene or a DNA fragment into a bacterial cell, which then multiplies (copies) the DNA when the cell divides. The basic steps involved in gene cloning is outlined below:
Step \(1\):
Step \(2\):
Step \(3\):
Step \(4\):
Step \(5\):
c. The first recombinant DNA was constructed by and in the year \(1972\).
d. A piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium was cut with the help of .