Answer the following question briefly:
Ethanol is an important organic compound used in industries, medicines, and fuels. It is produced on a large scale from by-products of sugar processing through a biochemical process involving microorganisms. The process includes preparation of the raw material, conversion of sugars, and purification to obtain a concentrated product. The stages and conditions involved determine the efficiency of ethanol production.
1. Arrange the following steps in the correct sequence for manufacture of ethanol from sugarcane.
1. Addition of yeast
2. Distillation of wash
3. Dilution of molasses
4. Addition of nitrogen source
1. Addition of yeast
2. Distillation of wash
3. Dilution of molasses
4. Addition of nitrogen source
2. Enzyme responsible for ethanol formation